Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells
Fig 5
Western blotting to determine Bid and t-Bid levels in Caco-2 cells (Top) and HT29 cells (Bottom).
Caco-2 and HT29 cells were treated with MA at IC50 and IC80 concentrations for 4 h. The levels of protein expression are expressed as arbitrary intensity units of each band compared to arbitrary intensity units of actin. MA produced clear effects on this protein in Caco-2 cells. However, this effect was not observable in HT29 cells. The values represent means ± SD. of at least three independent experiments performed in triplicate. Key: (*) p<0.05 and (**) p<0.01, with respect to the untreated cells.