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Regeneration of Vocal Fold Mucosa Using Tissue-Engineered Structures with Oral Mucosal Cells

Fig 6

Evaluation of an organotypic cultured vocal fold.

Hematoxylin and Eosin staining revealed that the tissue-engineered vocal fold mucosa consisted of the epithelium and lamina propria. The epithelium comprised two to four cell layers (A). Cells in the upper layer of the tissue-engineered vocal fold mucosa immunohistochemically expressed cytokeratin (B). Cells in the lower layer of the tissue-engineered vocal fold mucosa immunohistochemically expressed vimentin (C).

Fig 6

doi: https://doi.org/10.1371/journal.pone.0146151.g006