Flow Cytometry Approach to Quantify the Viability of Milk Somatic Cell Counts after Various Physico-Chemical Treatments
Fig 5
Cell viability with various centrifugation rates (400, 1500, 3000, 5000×g during 10 min at 4°C) comparing to the reference milk cell suspension without supplementary centrifugation (Ref).
(A) Boxplot and whiskers of flow cytometry results with milk somatic cell suspensions incubated with specific antibodies and vioblue live/dead kit (n = 4); (B) Cell viability of each type cell for 400×g (white bars), 1500×g (hatched bars), 3000×g (grey bars), 5000×g (dark bars) centrifugations by flow cytometry; (C) Mean proportion of macrophages (dark sectors), PMNs (grey sectors) and lymphocytes (white sectors) in cell samples under various centrifugation with different gravitational velocities. Means with different superscripts (a-d) differ significantly (P<0.05).