Catabolism of Branched Chain Amino Acids Contributes Significantly to Synthesis of Odd-Chain and Even-Chain Fatty Acids in 3T3-L1 Adipocytes
Fig 2
3T3-L1 adipocyte differentiation.
(A) Phase contrast images of 3T3-L1 cells from induction (day 0) to ten days post-induction. Cells initially display a fibroblast phenotype. Over the course of differentiation, cell morphology changes and cells accumulate lipid droplets internally. (B) Triglyceride staining of 3T3-L1 cells with Oil Red O. Initially, the fibroblast phenotype displays negligible staining. As the adipocytes mature, the amount of staining increases until almost the entire cell volume is stained red.