Modulation of Cytokine Secretion Allows CD4 T Cells Secreting IL-10 and IL-17 to Simultaneously Participate in Maintaining Tolerance and Immunity
Fig 6
Secretion of IL-10 but not of IL-17 is increased in the presence of sodium butyrate.
Pools of IL-10+ clones and IL-17+/IL-10+ clones (A) or pools of IL-17+ clones and IL-17+/IL-10+ clones (B) were stimulated with anti-CD3 and anti-CD28 alone or in combination or with PMA and ionomycin, alone or in combination, in the absence or in the presence of sodium butyrate. The levels of IL-10 (A) and IL-17 (B) secreted were assessed in the 24hrs culture supernatants, by ELISA. Data are shown as mean with SEM of duplicates. Data are representative of three independent experiments. Statistical analyses were performed using the Mann—Whitney U test. **p < 0.01, ***p < 0.001, ns, Non-significant.