Single-Tubed Wild-Type Blocking Quantitative PCR Detection Assay for the Sensitive Detection of Codon 12 and 13 KRAS Mutations
Fig 5
Sequencing results of WTB-PCR products from DNA samples containing various percentages of mutated KRAS alleles.
Sequencing chromatographs are shown for the products from the real-time traditional PCR (top row) and WTB-PCR (bottom row) reactions in Fig 4 and S3 Fig. The numbers at the bottom of each segment indicate the percentages of mutated KRAS c.35G>A allele in the PCR reaction mixtures. The arrows indicate the position of the G to A mutation of the KRAS c.35G>A allele.