Reduced Levels of Proteasome Products in a Mouse Striatal Cell Model of Huntington’s Disease
Fig 2
Representative MS data from peptidomics experiment comparing peptide levels between Q7Q7, Q7Q111 and Q111Q111 cells.
In this experiment, the two replicates of Q7Q7 cells were labeled with D0- and D3-TMAB-NHS, one replicate of the Q7Q111 cells was labeled with D9-TMAB-NHS and two replicates of Q111Q111 cells were labeled with D6- and D12-TMAB-NHS. A: Example of a peptide that is present in the Q111-expressing cell lines at levels much lower than in Q7Q7 cells. This peptide was identified by MS/MS analysis as TLLIKTVETRDGQVINETSQHHDDLE, derived from vimentin. B: Example of a peptide that is present in the Q111-expressing cell lines at levels slightly lower than in Q7Q7 cells. This peptide was identified by MS/MS analysis as Ac-MDTSRVQPIKLAR, derived from the 40S ribosomal protein S28. C: Example of a peptide that is present in all cell lines at comparable levels. This peptide was identified by MS/MS analysis as Ac-ADEIAKAQVAQPGGDTIFGKIIRK, derived from histidine triad nucleotide-binding protein 1.