Protein Kinase C-Independent Inhibition of Organic Cation Transporter 1 Activity by the Bisindolylmaleimide Ro 31-8220
Fig 5
Trans-inhibition of OCT1 activity by Ro 31–8220.
HEK-OCT1 cells were either untreated or exposed to 2 mM unlabelled TEA or 10 μM Ro 31–8220 for 1 h. After washing, cells were next incubated with 40 μM [14C]TEA for 5 min at 37°C. Intracellular accumulation of labelled TEA was then determined by scintillation counting. Data are expressed as % of accumulation of TEA found in untreated control cells, set at 100%, and are the means ± SEM of four independent experiments. *, p < 0.05 when compared with untreated cells (ANOVA followed by Dunnett's post-hoc test).