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Generation and Inheritance of Targeted Mutations in Potato (Solanum tuberosum L.) Using the CRISPR/Cas System

Table 2

Summary of targeted mutation screen of progeny from primary events and inheritance of Cas9.

Progeny from diploid (X46-3) and tetraploid (D46-9, D46-44) primary events were screened for inheritance of Cas9 (# of Cas9-free progeny) and Cas9-free progeny were screened for targeted mutations (# of Cas9-free progeny with mutations) (S6 and S7 Figs). Mutation transmission percentages are of Cas9-free progeny with targeted mutations and percent of Cas9-free are of the number of progeny screened. Mutations detected are targeted mutations cloned from primary events (F0) and progeny (F1).

Table 2

doi: https://doi.org/10.1371/journal.pone.0144591.t002