Generation and Inheritance of Targeted Mutations in Potato (Solanum tuberosum L.) Using the CRISPR/Cas System
Table 1
Summary of targeted mutation screen of primary events and enrichment PCR results from callus.
Diploid (X914-10) and tetraploid (Désirée) genotypes were stably transformed with gRNA746 and gRNA751 CRISPR/Cas reagents in a conventional 35S or geminivirus LSL T-DNA backbone using hygromycin selection (Total events). A restriction enzyme digestion assay and quantification of resistant and digested bands were used to identify events with at least 1% mutation frequencies (# with mutations) and events above a threshold using expected single allele mutation frequencies (# above threshold) (S2 Table). Percentages are of total events and modified enrichment PCR results come from Fig 1B and S1 Table.