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Y-27632, a ROCK Inhibitor, Promoted Limbal Epithelial Cell Proliferation and Corneal Wound Healing

Fig 2

Y-27632 promoted the proliferation of human limbal epithelial cells.

Phase contrast photographs of human limbal epithelial cells expanded on plastic dishes with or without adding various concentrations of Y-27632 in culture medium (A). Magnification: 200×. Cells with prominent nuclei and high nucleus/cytoplasm ratio as indicated by arrows were noted in dishes with Y-27632. There was a positive dose-dependent correlation between the concentrations of Y-27632 and proliferation of expanded limbal epithelial cells (B). Statistical analyses were performed using a paired test, and *p<0.05 was considered significant.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0144571.g002