Anti-Tumor Activity of Yuanhuacine by Regulating AMPK/mTOR Signaling Pathway and Actin Cytoskeleton Organization in Non-Small Cell Lung Cancer Cells
Fig 3
Effects of YC on the mTOR signaling pathway.
(A) H1993 cells were treated for 24 h with indicated concentrations of YC and the expressions of mTORC1 signaling-related proteins were measured by Western blot analysis. (B) H1993 cells were treated for 24 h with indicated concentrations of YC and the expressions of mTORC2 signaling-related proteins were measured by Western blot analysis. (C) The interaction of mTOR and rictor have been analyzed by immunoprecipitation of rictor with the following immunoblots of the cell lysates (lower panel) and immunoprecipitates (upper panel) with the indicated antibodies. IP: immunoprecipitation. (D) H1993 cells were treated for 24 h with the indicated concentrations of YC and the F-actin expression was measured by immunocytochemistry.