Anti-Tumor Activity of Yuanhuacine by Regulating AMPK/mTOR Signaling Pathway and Actin Cytoskeleton Organization in Non-Small Cell Lung Cancer Cells
Fig 2
Effects of YC on the AMPK signaling pathway.
(A) H1993 cells were treated for 24 h with the indicated concentrations of YC and the protein expressions were measured by Western blot analysis. (B) H1993 cells were treated for different short time points with 1 μM of YC and the protein expressions were measured by Western blot analysis. (C) H1993 cells were pretreated for 1 h with 20 μM of compound C, which is a well-known for AMPK inhibitor or 10 mM of metformin that is an AMPK activator. Then, 1 μM of YC was treated or co-treated for 2 h and the protein expressions were measured by Western blot analysis. (D) H358, H460, Calu-1, H1299, A549 and H1993 cells were treated for 2 h with 1 μM of YC and the protein expressions were measured by Western blot analysis.