Metabolic Conversion of Ceramides in HeLa Cells - A Cholesteryl Phosphocholine Delivery Approach
Fig 5
Analysis of C16-Cer precursor incorporation at different concentrations.
The ceramide was either (A) [3H]-labeled in the sphingosine backbone or in the (B) palmitic acid portion. After 24 h of ceramide uptake the total lipids were extracted and analysed by HPTLC. “Start” and “Front” refer to the labeled lipids remaining on the application spot, or that were eluted along the solvent front, “Other” refers to the traces of radiolabeled lipids between the identified spots (see S1 Fig). The data is from at least three different experiments.