Positive Allosteric Modulation of Kv Channels by Sevoflurane: Insights into the Structural Basis of Inhaled Anesthetic Action
Fig 5
Kv1.2 G329T recapitulates the magnified positive modulation of Kv1.2 FRAKT by sevoflurane.
(A) Effects of 1 mM sevoflurane on mutant whole-oocyte Kv1.2 currents evoked by a voltage step to +60 mV from a holding voltage of -100 mV. Black, red and grey current traces correspond to control, anesthetic-exposed, and washout, respectively. The scale bars indicate 50 ms and 1 μA. (B) Concentration-response relations of various general anesthetics acting on wild type and mutant Kv1.2 currents. Solid lines are the best fits assuming the double Hill equation (Materials and Methods). N = 4–8 oocytes for each dose. Best-fit parameters are summarized in Table 1.