Positive Allosteric Modulation of Kv Channels by Sevoflurane: Insights into the Structural Basis of Inhaled Anesthetic Action
Fig 2
Analysis of G-V relations from Kv1.2, ΔT1-Kv1.2, K-Shaw2, K-Shaw2 T330G and ΔT1-K-Shaw2.
(A) Best-fit Boltzmann parameters (V1/2, z and Gmax) from individual paired measurements before (Ctr) and after exposure to 1 mM sevoflurane (Sevo). Each pair of symbols connected by a solid line represents an individual paired experiment (Materials and Methods). The Gmax graphs depict raw values before normalization (in mS). The P value resulting from a paired Student-t test is shown above each graph, and the red marks indicate the mean values of the sample. (B)–(E) are as described for panel A. The number oocytes examined for each Kv channel was 6, 6, 4, 6 and 6, respectively.