MET Gene Amplification and MET Receptor Activation Are Not Sufficient to Predict Efficacy of Combined MET and EGFR Inhibitors in EGFR TKI-Resistant NSCLC Cells
Fig 2
The ERL-resistant HCC827 cells lines are tumorigenic.
A) Soft agar colonies were counted by ImageJ software. The percentage of colony forming efficiency (CFE) of parental (P) and ERL-resistant cell lines, as mean ± s.e.m., is shown. Histograms in gray and white colors indicate respectively vehicle (DMSO, 0.01%) and ERL (erlotinib 1 μM) cell treatments; B) Tumor volume of xenograft nodes, calculated as described in material and methods, are shown as mean ± s.e.m; C) Times to four (4x) and six fold (6x) increases from initial tumor volumes (day 3) are shown respectively by white and grey bars. P-values < 0.0001 are indicated.