Assessment of Interactions between Cisplatin and Two Histone Deacetylase Inhibitors in MCF7, T47D and MDA-MB-231 Human Breast Cancer Cell Lines – An Isobolographic Analysis
Fig 4
(A-F) Effects of HDIs and CDDP on caspase-3 activation in breast cancer cells.
Apoptotic changes induced by VPA or SAHA and CDDP alone or in combinations. Three breast cancer cell lines (T47D, MCF7, MDA-MB-231) were cultivated for 48 h with different doses of active drugs and their mixtures (2.0 = 100% IC50, 1.4 = 70% IC50, 1.0 = 50% IC50,) and analyzed by flow cytometry. The values present the percentage of the cells with active caspase-3. The results are depicted as means ± SD, n = 5 from 5 separate experiments. Statistical analysis was performed using one-way ANOVA test (*** p<0.001, ** p<0,01, * p<0,05).