Establishment of Green Fluorescent Protein and Firefly Luciferase Expressing Mouse Primary Macrophages for In Vivo Bioluminescence Imaging
Fig 5
Reporter macrophages in in vivo bioluminescence imaging.
Lentivirus and cyclosporine transduced reporter macrophages where injected into the tail vein of mouse model of biomaterial induced local inflammation and osteolysis. The cell trafficking was followed by bioluminescence imaging. Luminescence emanating from the local area of inflammation in the right distal femur as well as left distal femur serving as a control was determined from images obtained every other day up to 20 days post injection. a) Bioluminescence images showing the accumulation of reporter cells to the right distal femur starting on the day 2 after the systemic injection of the reporter cells and persisting over the 20 day imaging period. b) XY-blot showing the total flux from regions of interest over the right and left distal femurs. * = p > 0.05; ** = p > 0.01, *** = p > 0.001.