Compensatory Response by Late Embryonic Tubular Epithelium to the Reduction in Pancreatic Progenitors
Fig 5
P1 transgenic pancreases contain significantly larger endocrine clusters compared to E17.5.
At P1 bigenic islets had relatively normal organization as compared to controls for expression of glucagon (green, AB, red, EF), somatostatin (green, CD), pancreatic polypeptide (green, EF), ghrelin (green, GH) and insulin (red, A-D, G-J). DBA-expressing branching ducts were observed in both neonates (green, IJ). DAPI (blue). Bar: 50 μm. Quantification of these data show increases in the insulin+ and glucagon+ area of bigenic pancreas from E17.5 to P1 compared to controls (KL). Total pancreatic area in Pdx1tTA/+;tetOMafA also shows enhanced growth (M). Blood glucose levels from each group of neonates (n = 8 for each group) show that bigenic neonates were significantly hyperglycemic at P1 (N). Mean ± s.e.m.