Activation of AMP-Activated Protein Kinase by Adenine Alleviates TNF-Alpha-Induced Inflammation in Human Umbilical Vein Endothelial Cells
Fig 5
Effects of adenine on monocyte adhesion to HUVECs in response to TNF-α.
shNT HUVECs or shAMPK HUVECs were treated with 10 μg/L of TNF-α for 6 h in the presence or absence of 600 μM adenine. Top: representative images depicting monocytes to the HUVEC cells as described in “Material and methods”, Scale bar: 200 μM. Bottom: quantification of monocyte adhesion to HUVEC cells. Statistical significance was determined by one-way ANOVA followed by Tukey post hoc tests; all data are plotted as mean ± S.E.M. (n = 5). ***, P<0.001; N.S., no significance.