Antagonistic Regulation of Parvalbumin Expression and Mitochondrial Calcium Handling Capacity in Renal Epithelial Cells
Fig 5
Silencing of ectopic PV in MDCK cells (clone PV15).
A) Down-regulation of Pvalb mRNA via constitutive lentiviral-mediated shRNA decreases PV protein expression levels after 10 days of puromycin selection. B) PV down-regulation is less potent via the IPTG-inducible shRNA MISSION® system tested in the PV-positive MDCK clone PV15. C) IHC of PV clone PV15 infected with constitutive lentiviral shPvalb after 10 days of puromycin selection (right image) in comparison to untreated cells (left image).