dRNA-Seq Reveals Genomewide TSSs and Noncoding RNAs of Plant Beneficial Rhizobacterium Bacillus amyloliquefaciens FZB42
Fig 3
A subset of operons antisense to known genes.
Two operons identified antisense to a known operon (A) or to known genes (B). cDNA reads in each library without (-TEX) or with (+TEX) terminator exonuclease treatment were mapped to B. amyloliquefaciens FZB42 chromosome and visualized by IGB 8.1. Vertical bars indicate cDNA recovery detected in each library and were adjusted to the same scale of 20. The genes indicated above the horizontal coordinates are located on the forward strand while the genes indicated below the horizontal coordinates are located on the reverse strand. Bacterial cultures were sampled for total RNA extraction at two time points (#6h	h) from four conditions: i) in 1CM medium; ii) 1CM medium supplemented with the maize root exudates (+RE); iii) 1CM medium supplemented with soil extract (+SE); iv) 1CM medium supplemented with both maize root exudates and soil extract (+RS). The time point #6h corresponded to early stationary phase while #9h corresponded to middle stationary phase.