FL3, a Synthetic Flavagline and Ligand of Prohibitins, Protects Cardiomyocytes via STAT3 from Doxorubicin Toxicity
Fig 6
Small-interfering-RNA (siRNA)-mediated downregulation of PHB1 proteins attenuates FL3-induced cardioprotection from doxorubicin toxicity.
A. Representative western blots show induction of STAT3 phosphorylation by the synthetic flavagline (FL3) in H9c2 cells transfected with nonspecific siRNA (left) or with anti-PHB1 siRNA (right). B. Quantification of phosphorylated STAT3, with normalization to actin (n = 3; *p < 0.05, compared to vehicle). FL3-mediated STAT3 activation by phosphorylation was abolished when the expression of PHBs were reduced. C. This histogram shows downregulation of PHB1 after transfection of H9c2 cells with anti-PHBs siRNA (n = 3; *p < 0.05, compared to vehicle). D. This histogram shows downregulation of PHB2 after transfection of H9c2 cells with anti-PHBs siRNA (n = 3; *p < 0.05, compared to vehicle).