FL3, a Synthetic Flavagline and Ligand of Prohibitins, Protects Cardiomyocytes via STAT3 from Doxorubicin Toxicity
Fig 3
The mitochondrial STAT-3 phosphorylation is correlated with PHB1 translocation to mitochondria by FL3 in cardiomyocytes.
A and B. Representative western blot analyses and histogram based quantification of mitochondrial STAT3 activation by phosphorylation. STAT3 was phosphorylated by FL3 in mitochondrial fraction. C and D. Representative western blot analyses and histogram based quantification of nuclear STAT3 activation by phosphorylation. STAT3 activation was only detected in nucleus after FL3 treatment. E and F. The western blot and histogram show quantitative analyses of nuclear phosphorylated STAT3 levels upon treatment of H9c2 cells with control (DMSO), FL3 (100 nM), doxorubicin (1μM), and FL3 + doxorubicin. Doxorubicin elevated phosphorylated STAT3 levels, which were reduced by FL3 (n = 3; *p < 0.05, compared to vehicle; **p < 0.05, compared to doxorubicin treatment).