FL3, a Synthetic Flavagline and Ligand of Prohibitins, Protects Cardiomyocytes via STAT3 from Doxorubicin Toxicity
Fig 1
Synthetic flavagline (FL3) binds PHB1 and PHB2 and increases PHB1 leves in H9c2 cells.
A. Whole-cell extracts of the H9c2 line (input) were either incubated with the beads Affi-Gel 10 conjugated with FL3 or blocked with ethanolamine (unconjugated Affi-Gel) [7,17]. The bound and eluted proteins (Affi-Gel-FL3) and output proteins (output Affi-Gel-FL3) were analyzed by western blotting using antibodies against PHB1 and PHB2 (n = 3). B and C. Representative western blot analyses and histogram based quantification of total PHB1 levels in the cell lysates by. FL3 alone increased PHB1 protein levels within 10h as compare to non-treated cells (NT). However PHB1 level was lower in the present of both FL3 and doxorubicin (n = 3). D and E. Representative western blot analyses and histogram based quantification of nuclear PHB1 levels by. Doxorubicin accumulates PHB1 in the nucleus but lowers in the cytoplasm that was reduced by preconditioning with FL3. * Indicates p<0.05 as compare to control, ** indicates p<0.05 as compare to the doxorubicin alone group.