Non-Invasive Microbial Metabolic Activity Sensing at Single Cell Level by Perfusion of Calcein Acetoxymethyl Ester
Fig 10
Metabolic activity sensing of cells exposed to unviable antibiotic concentrations and metabolic activity changes of descendants after antibiotic stress.
After an initial growth phase in complex medium BHI, cells were exposed to (A) 10 μg/mL ampicillin (cell wall inhibition, n = 5 colonies) and (B) 10 μg/mL chloramphenicol (inhibition of protein synthesis, n = 5 colonies), respectively, for one hour. The antibiotics were added to the perfusion medium during the exposure time and after an hour perfusion with antibiotic free BHI was continued. Calcein mean single cell fluorescence revealed how the antibiotics change the bacterial fitness during antibiotic exposure. (C) The apparent growth rate was determined for five microcolonies treated for one hour with AMP or CHL.