Non-Invasive Microbial Metabolic Activity Sensing at Single Cell Level by Perfusion of Calcein Acetoxymethyl Ester
Fig 5
Mean CAM conversion rate constant and mean calcein efflux rate constant.
(A) A mean maximal single cell reaction rate constant at 0.0025 min-1 was determined for CAM conversion under inhibition of calcein efflux due to carbon limitation. A maximal mean single cell calcein efflux rate constant was determined to be approximately twice as high at 0.005 min-1 (n = 5 colonies for each mean maximal single cell reaction rate constant). (B) Limitations in carbon supply inhibited cell division and energy driven transport of calcein out of the cell. Thus, mean single cell traces increase linearly over time until carbon supply was continued (every colored dotted line represent one individual cell, n = 15 cells).