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Non-Invasive Microbial Metabolic Activity Sensing at Single Cell Level by Perfusion of Calcein Acetoxymethyl Ester

Fig 1

Microfluidic device and its submicrostructures.

(A) Assembled microfluidic device with connected inlet and outlet tubings. (B) Parallel cultivation chamber arrays with branched main channels that subdivide in smaller channels for media perfusion. A a single device contains four cultivation chamber arrays with 352 chambers each. (C) Microcultivation chamber. (D) SEM micrograph of a cultivation chamber.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0141768.g001