Tumor-Associated Endothelial Cells Promote Tumor Metastasis by Chaperoning Circulating Tumor Cells and Protecting Them from Anoikis
Fig 5
Endothelial cells expressing (EC-Bcl-2) protect tumor cells from anoikis via the Src-FAK signaling pathway.
A-C; CAL27 cells were co-cultured along with EC-VC or EC-Bcl-2 cells in a non-adherent 6 well plates for 8 hours. At the end of incubation, cells were carefully retrieved and analyzed for anoikis by TUNEL staining (A) or cell lysate was prepared and Western blotted for pSrc, pFAK, Src and FAK (B). C; tumor cell anoikis assay was performed by either adding neutralizing anti-E-selectin antibody or knocking down FAK in tumor cells.