Qualitative and Quantitative Analysis of ROS-Mediated Oridonin-Induced Oesophageal Cancer KYSE-150 Cell Apoptosis by Atomic Force Microscopy
Fig 5
ROS scavenger-NAC reversed oridonin induced oesophageal cancer KYSE-150 cell membrane ultrastructural changes.
AFM morphology and membrane ultrastructure imaging of (A) control, (B) 10 μM oridonin treated, (C) 30 μM oridonin treated, (D) 50 μM oridonin treated, (E) 2.5 mM NAC+50 μM oridonin treated and (F) 2.5mM NAC treated KYSE-150 cells. (A1-F1) Topogrphy images of KYSE-150 cells, scale bar: 20 μm; (A2-F2) Enlarged membrane ultrastructure images in (A1-F1) and (A3-F3) their corresponding 3D images of KYSE-150 cells, scale bar: 500 nm; (A4-F4) Height distribution and roughness of cell surface ultrastructure analyzed from (A2-F2).