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Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding

Fig 5

J-Plots and CD spectra of γ-ce-H-(T₁₀)-Gly-NH₂ (2)/dA₁₀.

(A) Absorbance of the γ-ce-H-(T₁₀)-Gly-OH (2)/dA₁₀ mixtures with various PNA/DNA ratios at 260 nm; (B) Absorbance of the mixtures at 275 nm; (C) Molar ellipticity at 263 nm. Conditions: 10 mM Na₂HPO₄ (pH 7.4), 140 mM KCl, 5 mM MgCl₂. The summed PNA and DNA concentration was 7 μM at each ratio; (D) CD spectra of the triplex. The molar ellipticity is given per 1 nucleotide in a strand. Conditions: 10 mM Na₂HPO₄ (pH 5 or 7.4), 140 mM KCl, 5 mM MgCl₂. The triplex concentration was 1.8 μM.

doi: https://doi.org/10.1371/journal.pone.0140468.g005