Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding
Fig 4
UV-melting and CD spectra of PNA/DNA and DNA/DNA complexes.
(A) Thermal difference spectra of the complexes; (B) Thermal denaturation profiles of the complexes; (C) CD spectra of the single-stranded oligonucleotides and PNAs. The molar ellipticity was calculated per 1 nucleotide. (D) CD spectra of the complexes. Conditions: 10 mM Na2HPO4 (pH 7.4), 140 mM KCl, 5 mM MgCl2. The concentration of each PNA or oligonucleotide was 2.5 μM. The CD spectra were measured at 15°C.