The Structural Differences between a Glycoprotein Specific F-Box Protein Fbs1 and Its Homologous Protein FBG3
Fig 5
In vitro RNase B binding activities of the Skp1–FBG3 complex and its mutants using pull-down assay.
(A) Determination of the crucial loops for the carbohydrate-binding pocket formation in FBG3. (B) Comparison of relative RNase B binding activities between Skp1–Fbs1 wild type (control) and Skp1–FBG3 loop mutants shown in (A). Three independent pull-down assays were analyzed. Error bars represent means ± S.E.