Malva sylvestris Inhibits Inflammatory Response in Oral Human Cells. An In Vitro Infection Model
Fig 4
Gene expression analysis of lower chamber co-culture invasion assay.
The expression levels of IL-1alfa, IL-1beta, IL-6, IL-8, IL-19, CD14, PTGS, MMP-1, FOS, BCL2 were evaluated and compared to the control (non-infected cells). Quantification of the relative transcript amounts was performed using qPCR with 50 ng of each cDNA. Data quantification was performed using the 2ΔΔCT method. Statistical analyses included one-way ANOVA followed by Dunnet’s post-hoc tests. A significance level of p < 0.05 (*) indicates differences from the vehicle group.