Circadian Clock Genes Are Essential for Normal Adult Neurogenesis, Differentiation, and Fate Determination
Fig 2
NSPCs are the dominant cell types during the first four days of DG neurosphere differentiation.
SOX2+ (green) cells after A: 1 day in SM, B: 4 days in SM. Msi1+ (green) cells after C: 1 day in SM and D: 4 days in SM. E: Nestin+/GFAP+(yellow) cells at day 4 in SM indicating that radial glial-like cells persist after 4 days of differentiation in SM. Also shown are Nestin+/GFAP- (green) cells and very few Nestin-/GFAP+ (red) cells. Neurospheres lack immature and mature neuronal cells after differentiating 4 days in SM as shown by F: BetaIII+ (green) and G: NeuN+ (green). Nuclei were stained with propidium iodide (red). H: The source of the bioluminescence signal indicated by mPER1+ (green) cells in the neurosphere core after 3 days in SM. All nuclei were stained with Hoechst3342 (blue) unless specified. Scale bar = 50 μm.