New Insights into Butyrylcholinesterase Activity Assay: Serum Dilution Factor as a Crucial Parameter
Fig 4
Characteristics of fluoride inhibition of “usual” and “fluoride” forms of BChE.
FN values were calculated in assays performed at various dibucaine concentration at 25°C using 5mM BTC (A) or PTC (B) as substrate with two types of 400-fold diluted serum samples from heterozygous individuals with usual UU and UF phenotype. Fluoride Number (FN) = (1 - (butyrylcholinesterase activity in presence of inhibitor/butyrylcholinesterase activity in absence of inhibitor)) x 100. Assays were performed in triplicate, standard error for all points is less than 3% of the values.