TMPyP4, a Stabilizer of Nucleic Acid Secondary Structure, Is a Novel Acetylcholinesterase Inhibitor
Fig 3
TMPyP4 inhibits AChE activity.
A, oxidation of heme by Hemeoxygenase (HO) enzymes produces biliverdin and carbon monoxide. B, oxidation of TMPyP4 by HO enzymes is expected to generate either 4-formyl-1-methylpyridinium (4F-MP) or 4-carboxy-1-methylpyridinium (4C-MP). Both 4F-MP and 4C-MP have a structural resemblance to acetylcholine. C, fluorometric assay of AChE activity in presence of TMPyP2, TMPyP4, 4F-MP, 4C-MP and an established AChE inhibitor (Donepezil). D, pre-incubation of TMPyP4 with recombinant HO-2 prior rescued AChE activity in a concentration-dependent manner.