Genetic Ablation of the ClC-2 Cl- Channel Disrupts Mouse Gastric Parietal Cell Acid Secretion
Fig 5
(A) Effect of histamine on the pH of gastric contents and (B) & (C) effect of histamine/carbachol on acid (B) and pepsinogen (C) secretion in WT and ClC-2-/- mouse gastric mucosa.
(A) The pH of gastric contents was measured in WT (white column) and ClC-2-/- (black column) mice after 15 min of histamine stimulation. Data are plotted as mean ± SE (n = 3). *P<0.025 versus WT. For (B) & (C) mouse stomachs were perfused and at 30 min subcutaneous histamine/diphenhydramine (HIST, 0.23 mg/h/DPH, 0.03 mg/h) and intraluminal carbachol (CCH, 0.5 mg/ml) were started. 15 min samples of gastric effluent were collected and acid, pH and pepsinogen were measured. Data are plotted as mean ± SE. For (B) WT n = 11 & ClC-2-/- n = 8, *P<0.005 versus WT. For (C) WT & ClC-2-/- n = 7, NS, not significant versus WT.