Genetic Ablation of the ClC-2 Cl- Channel Disrupts Mouse Gastric Parietal Cell Acid Secretion
Fig 2
Immunolocalization and expression of H/K ATPase-β subunit in WT and ClC-2-/- mouse gastric mucosa.
Gastric mucosal sections from WT and ClC-2-/- mice were stained for H/K ATPase β subunit by immunohistochemistry (A) and immunofluorescence (C). H/K ATPase β subunit positive cells were orange/brown in (A) and green in (C). Bar in (A) = 25 μm; bar in (B) = 10 μm, representative figures from n = 10–20 regions examined. (B) Quantitation of H/K ATPase-containing cells/gland of WT and ClC-2-/- gastric mucosa, n = 6. #P<0.05 versus WT. (D) Western blot of H/K ATPase in WT and ClC-2-/- mouse gastric mucosa, with β-actin as loading control. (E) Quantitation of H/K ATPase western blot by densitometry, normalized to β-actin, n = 3. *P<0.001 versus WT. Data in (B) and (E) are plotted as mean ± SE.