dsdA Does Not Affect Colonization of the Murine Urinary Tract by Escherichia coli CFT073
Fig 2
Inactivation of dsdA does not engender a competitive advantage in CBA/J mice during experimental UTI.
CFT073 ΔlacZYA rpoSam and CFT073 ΔdsdA rpoSam or CFT073 dsdAΔ445bp gyrAS83L or CFT073 gyrAS83L were co-inoculated at a 1:1 ratio into CBA/J mice (n = 16, 8, and 18, respectively). Mice were sacrificed at 48hpi. Bacteria from (A) bladder and (B) kidney homogenates were enumerated on MacConkey’s medium plus lactose. Several mice from the co-infections had no detectable bacteria in their kidneys (4 of 16, 5 of 8, and 3 of 18, respectively). Lines are drawn at the geometric mean relative competitive index (RCI). Statistical Significance was assessed by a Wilcoxon signed-rank test relative to a hypothetical RCI of 1.