Scanning MscL Channels with Targeted Post-Translational Modifications for Functional Alterations
Fig 9
Functional changes by substitutions in the TM2 domain of MscL, determined by in vivo and patch clamp experiments.
(A) The location of the residues showing changes in viability upon post-translational modifications is highlighted in the closed structure of E. coli MscL. A pentameric MscL is shown side view where the approximate location of the membrane marked, followed by single subunit with the labeled residues. Cytoplasmic and periplasmic views are also shown below. (B) Viability of MJF 367 (mscL-) cells, shock in the presence of the indicated MTS reagent is shown for each individual MscL, for hydrophobic or C. charged MTS reagents.