Scanning MscL Channels with Targeted Post-Translational Modifications for Functional Alterations
Fig 7
Functional changes by substitutions in the periplasmic loop of MscL, determined by in vivo and patch clamp experiments.
(A) The location of the residues showing changes in viability upon post-translational modifications is highlighted in the closed structure of E. coli MscL. From right to left: a pentameric MscL is shown in cytoplasmic view, a periplasmic view, and a side view where the approximate location of the membrane is shown. A single subunit and a close up of the region with the labeled residues is on the left. (B) Viability of MJF 367 (mscL-) shock in the presence of the indicated MTS reagent is shown for each individual MscL mutant, for hydrophobic or (C) charged MTS reagents.