Anti-Inflammatory Effects of IL-27 in Zymosan-Induced Peritonitis: Inhibition of Neutrophil Recruitment Partially Explained by Impaired Mobilization from Bone Marrow and Reduced Chemokine Levels
Fig 3
IL-27 pre-treatment decreases neutrophil accumulation in the murine blood and temporarily in the bone marrow during zymosan-induced peritonitis.
Dot plots show the gating for neutrophils in the FACS analyses of blood cells and bone marrow cells (at at 12h and 4h after zymosan injection, respectively). Cell frequencies within the gate are given for the exemplary FACS plots. A. Absolute numbers of blood neutrophils in the blood of vehicle-treated animals (vehicle) 12h after injection and animals with or without pre-treatment with rIL-27 monitored 4–48h after injection of zymosan. Results from four different experiments are combined; given are means ± S.E.M (n = 8–11). B. Absolute numbers of neutrophils in the bone marrow of vehicle-treated animals (12h after vehicle injection) and 4–48h after injection of zymosan with or without pre-treatment with rIL-27. Results from four different experiments are combined; given are means ± S.E.M (n = 8–11). Mann–Whitney test was used to compare between groups, *p<0.05, ** p<0.01, ***p<0.005.