Anti-Inflammatory Effects of IL-27 in Zymosan-Induced Peritonitis: Inhibition of Neutrophil Recruitment Partially Explained by Impaired Mobilization from Bone Marrow and Reduced Chemokine Levels
Fig 1
Influence of IL-27 on the inflammatory response in sterile zymosan-induced peritonitis.
IL-27 was either given together with zymosan (zymosan +IL-27) or 12 h before zymosan application into the peritoneum (zymosan +preIL-27). The control group received zymosan only. A. Representative FACS plots for peritoneal neutrophils and macrophages isolated from the peritoneal lavage 12h after induction of peritonitis. GR1+CD11b+ cells are defined as neutrophils, CD11b+ single positive cells as macrophages. Cell frequencies within the neutrophil gate are depicted. B. Absolute number of neutrophils in the peritoneal lavage at various time points after zymosan injection. Results are pooled from four independent experiments and represent mean ± S.E.M (n = 4–12). C. Absolute number of macrophages in the peritoneal lavage at various time points after zymosan injection. Pooled data from four independent experiments, mean ± S.E.M. (n = 4–12). Mann–Whitney test was used to compare between groups, *p<0.05, ** p<0.01.