Enhanced Expression of Integrin αvβ3 Induced by TGF-β Is Required for the Enhancing Effect of Fibroblast Growth Factor 1 (FGF1) in TGF-β-Induced Epithelial-Mesenchymal Transition (EMT) in Mammary Epithelial Cells
Fig 5
FGF1 augments TGF-β1-induced loss of polarity depends on the interaction between FGF1 and integrin αvβ3.
A and B, MCF10A cells were seeded on glass cover slip coated with growth factor-reduced Matrigel. After 2 weeks of incubation, acinar structures were formed on the Matrigel. Assay medium was replaces to DMEM/F12 containing 2% horse serum and 2% growth factor-reduced Matrigel. Then cells were treated for inducting EMT with 5 ng/ml TGF-β1 together with either 50 ng/ml WT-FGF1 (WT) or integrin binding defective FGF1 mutant (R50E) for 4 days. The acini were immunostained with an anti-integrin αvβ3 (A, green) or an anti-integrin α6 (B, green) antibody together with DAPI labels nuclei (blue). Confocal images were acquired at the center plane of the acinar structure. Scale bar = 50 μm. C, MCF10A cells were treated as in A, then acini indicating discontinuous basement membrane was counted from the images.