Mechanisms of Dihydroartemisinin and Dihydroartemisinin/Holotransferrin Cytotoxicity in T-Cell Lymphoma Cells
Fig 2
HTF enhanced ROS production following DHA exposure.
Jurkat cells (1 × 106/well) were exposed to DHA (0, 10, 20, 40, 80 μM) or DHA/HTF for 48 h. The fluorescent probe DCFH-DA identified a dose-dependent increase in ROS following exposure to DHA. Furthermore, combined exposure of Jurkat cells to 20 μM DHA and 20 μM HTF produced over three-fold more ROS than exposure to 20 μM DHA alone (p < 0.001). The results represented mean ± standard deviation. ***p<0.001, compared with the control group, # p<0.001, compared with DHA.