Melanin Transfer in Human 3D Skin Equivalents Generated Exclusively from Induced Pluripotent Stem Cells
Fig 5
3D skin equivalents produced from iPSC-derived fibroblasts, keratinocytes and melanocytes have normal architecture and are functional.
a) Hematoxylin and eosin, b) Ki67, c) gp-100, d) Fontana-Masson, e) loricrin, f) keratin-1, g) keratin-14. Blue (DAPI), dashed line (basement membrane). Inset c) (enlarged image showing an iPSC-derived melanocyte in the basal layer of the epidermis). Inset d) (enlarged image showing an iPSC-derived melanocyte producing melanin). h & i) Control and forskolin-treated iPSC-derived 3D skin equivalents, respectively, after 14 days at the air-liquid interface. j) Quantification of melanin increase in 3D skin equivalents in response to 40μM forskolin. Scale bar = 100μm (except in enlarged images = 10μm). *** = p<0.001.