A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas
Fig 5
Isolation of IgG ɣ2a antibody secreting cells from an IgG ɣ2b hybridoma cell line.
(A) Detection of IgG ɣ2a antibody secreting hybridoma cells in S212, an IgG ɣ2b hybridoma cell line with ELISPOT. (B) Cell surface IgG staining of S212 cells. (C) Enrichment of IgG ɣ2a secreting cells by MACS and single cell sorting of these heavy chain switched cells. (D) Biacore testing of S212 IgG ɣ2b antibody and the switched IgG ɣ2a antibody.