Acute Treatment with a Novel TRPC4/C5 Channel Inhibitor Produces Antidepressant and Anxiolytic-Like Effects in Mice
Fig 3
Distribution of M084 in serum, brain and CSF of mice.
Serum, brain, and CSF samples were collected at 2 hours after treatment of M084 or vehicle. Concentrations of M084 were determined with LC-MS/MS analysis. (A) M084 can efficiently cross the blood-brain barrier at pharmacologically relevant doses (10 mg/kg, 2 hrs). Results are shown as mean ± SEM. (B) M084 calibration curve. Correlation coefficient = 0.999654, weighting factor = 1/x. (C-F) Representative LC–MS/MS chromatogram of blank brain sample, serum, brain and CSF, respectively.